Ultra-Widefield and High-Speed Spiral Laser Scanning OR-PAM: System Development and Characterization.

Mohsin ZafarRayyan ManwarLaura S McGuireFady T CharbelKamran Mohammad Avanaki

Published in: Journal of biophotonics (2023)

Photoacoustic microscopy (PAM) is a high-resolution imaging modality that has been mainly implemented with small field of view applications. Here, we developed a fast PAM system that utilizes a unique spiral laser scanning mechanism and a wide acoustic detection unit. The developed system can image an area of 12.5cm 2 in 6.4s. The system has been characterized using highly-detailed phantoms. Finally, the imaging capabilities of the system were further demonstrated by imaging a sheep brain ex-vivo and a rat brain in-vivo.

Keyphrases

high resolution
high speed
atomic force microscopy
mass spectrometry
machine learning
single molecule
high throughput
label free
subarachnoid hemorrhage