Red Fluorescence Conjugated Polymer with Broad Spectrum Antimicrobial Activity for Treatment of Bacterial Infections In Vivo.

To address the problem of bacterial resistance, a practical strategy for broad spectrum antimicrobial based on conjugated polymers was proposed in the work. Three red fluorescence conjugated polymers (P1, P2, and P3) bearing quaternary ammonium groups with different length of side chains were designed and synthesized. By virtue of inserting capacity of the longer side chain, conjugated polymer (P3) displayed well broad spectrum antimicrobial activity toward Gram-negative and Gram-positive bacteria and fungi under a white light density of 25 mW cm-2 and short time (15 min) by aid of dark toxicity and light toxicity, derived from the quaternary ammonium groups and reactive oxygen species produced by the backbone, respectively. Notably, for ampicillin-resistant Escherichia coli TOP10, P3 could kill the bacteria 100% at a very low concentration of 5 μM upon light irradiation. Furthermore, wound healing tests indicated that the polymer could be expediently employed for wound disinfection in vivo without any tissue damaging. The contribution of the work not only provides an efficient and broad spectrum antimicrobial material but also offers a multimodal antimicrobial strategy to fight against bacterial infections in vivo.