Internal implants are widely used in most orthopedic surgeries, of which titanium and its alloys are most widely used owing to the excellent corrosiveness resistance, low elastic modulus and good biocompatibility. However, implant failure still occurs for that titanium and its alloys themselves do not own antibacterial and osteogenic properties. In this work, we successfully fabricated berberine-loaded graphene oxide (GO) on the surface of biomedical titanium and systematically investigated its capabilities of antibacteria and osteogenesis. In vitro results showed that berberine had low antibacterial activity, but GO loaded with berberine on titanium (Ber&GO@Ti) exhibited superior antibacterial activity against Staphylococcus aureus ( S. aureus ) with the synergistic effect of GO and berberine. Meanwhile, Ber&GO@Ti performed satisfactory cytocompatibility and was capable of promoting osteogenic differentiation of MC3T3-E1 cells. In the vivo experiment, Ber&GO@Ti showed excellent antibacterial properties and inflammatory cells e.g., neutrophils had seldom been found. No visceral toxicity had been found. This multifunctional coating showed great potential in orthopedic implants.
Keyphrases
- drug delivery
- silver nanoparticles
- cancer therapy
- induced apoptosis
- wound healing
- staphylococcus aureus
- cell cycle arrest
- mesenchymal stem cells
- oxidative stress
- bone marrow
- soft tissue
- anti inflammatory
- metabolic syndrome
- cell death
- escherichia coli
- risk assessment
- insulin resistance
- essential oil
- pseudomonas aeruginosa
- high resolution
- methicillin resistant staphylococcus aureus
- biofilm formation