Firefly luciferin methyl ester illuminates the activity of multiple serine hydrolases.

Innus MohammadKate L LiebmannStephen C Miller

Published in: Chemical communications (Cambridge, England) (2023)

Firefly luciferin methyl ester is hydrolyzed by monoacylglycerol lipase MAGL, amidase FAAH, poorly-characterized hydrolase ABHD11, and hydrolases known for S -depalmitoylation (LYPLA1/2), not just esterase CES1. This enables activity-based bioluminescent assays for serine hydrolases and suggests that the 'esterase activity' responsible for hydrolyzing ester prodrugs is more diverse than previously supposed.

Keyphrases

protein kinase
high throughput