Miuramides A and B, Trisoxazole Macrolides from a Mycale sp. Marine Sponge That Induce a Protrusion Phenotype in Cultured Mammalian Cells.
Rei SuoKentaro TakadaHisanori KohtsukaYuji IseShigeru OkadaShigeki MatsunagaPublished in: Journal of natural products (2018)
Morphology-guided cell-based screening of the extract of a Mycale sp. marine sponge led to the isolation of two trisoxazole macrolides, miuramides A (1) and B (2), which induced characteristic morphological changes in 3Y1 cells. The structure of 1 including absolute configuration was elucidated by a combination of the analysis of spectroscopic data, derivatization, and degradation. Both compounds exhibit potent cytotoxicity against 3Y1 cells.
Keyphrases
- induced apoptosis
- cell cycle arrest
- oxidative stress
- endothelial cells
- single cell
- endoplasmic reticulum stress
- machine learning
- anti inflammatory
- diabetic rats
- high glucose
- electronic health record
- gas chromatography mass spectrometry
- high performance liquid chromatography
- bone marrow
- tandem mass spectrometry
- molecular dynamics simulations
- ultra high performance liquid chromatography