Cell surface heat shock protein-mediated entry of tumor cell-adapted rotavirus into U-937 cells.
José RicoClaudia PerezJuan HernandezCarlos A GuerreroOrlando AcostaPublished in: Folia microbiologica (2021)
Rotaviruses infect cells by binding to specific cell surface molecules including gangliosides, heat shock protein cognate protein 70 (Hsc70), and some integrins. The characterization of cell surface receptors defining viral tropism is crucial for inhibiting entry into the normal cells or the cancer cells. In the present work, several tumor cell-adapted rotavirus isolates were tested for their interaction with some heat shock proteins (HSPs) present in the U-937 cells, derived from a human pleural effusion (histiocytic lymphoma monocyte). This interaction was examined by virus overlay protein-binding (VOPB), immunochemistry, immuno-dot blot assays, and flow cytometry. The results indicated that the rotavirus isolates studied were able to infect U937 cells by interacting with Hsp90, Hsp70, Hsp60, Hsp40, Hsc70, protein disulfide isomerase (PDI), and integrin β3, which are implicated in cellular proliferation, differentiation, and cancer development. Interestingly, these cellular proteins were found to be associated in lipid microdomains (rafts), facilitating in this way eventual sequential interactions of the rotavirus particles with the cell surface receptors. The rotavirus tropism for U937 cells through the use of these cell surface proteins made this rotavirus isolates an attractive target for the development of oncolytic strategies in the context of alternative and complementary treatment of cancer.
Keyphrases
- heat shock protein
- cell surface
- heat shock
- induced apoptosis
- cell cycle arrest
- signaling pathway
- squamous cell carcinoma
- heat stress
- endoplasmic reticulum stress
- single cell
- endothelial cells
- stem cells
- amino acid
- sars cov
- young adults
- small molecule
- cell proliferation
- mesenchymal stem cells
- papillary thyroid
- smoking cessation
- cell therapy
- peripheral blood
- genetic diversity
- quantum dots