A Biosensor for Detection of Indole Metabolites.
Jiefei WangChao ZhangW Seth ChildersPublished in: ACS synthetic biology (2021)
Microbially produced indole metabolites serve as a diverse family of interspecies and interkingdom signaling molecules in the context of human health, crop production, and antibiotic resistance. We mined the protein database for sensors of indole metabolites and developed a biosensor for indole-3-aldehyde (I3A). Microbially produced I3A has been associated with reducing inflammation in diseases such as ulcerative colitis by stimulating the aryl hydrocarbon receptor pathway. We engineered an E. coli strain embedded with a single plasmid carrying a chimeric two-component system that detects I3A. Our I3A receptor characterization confirmed binding site residues that contribute to the sensor's I3A detection range of 0.1-10 μM. This new I3A biosensor opens the door to sensing indole metabolites produced at various host-microbe interfaces and provides new parts for synthetic biology applications.
Keyphrases
- label free
- ms ms
- human health
- sensitive detection
- gold nanoparticles
- risk assessment
- escherichia coli
- ulcerative colitis
- quantum dots
- climate change
- loop mediated isothermal amplification
- oxidative stress
- cell therapy
- real time pcr
- stem cells
- emergency department
- binding protein
- mesenchymal stem cells
- low cost
- adverse drug