A GFP Inspired 8-Methoxyquinoline-Derived Fluorescent Molecular Sensor for the Detection of Zn 2+ by Two-Photon Microscopy.
Attila CsomosMiklós MadarászGábor TurczelLevente CseriAndrea BodorAnett MatuscsákGergely KatonaErvin KovácsBalázs J RózsaZoltan MucsiPublished in: Chemistry (Weinheim an der Bergstrasse, Germany) (2024)
An effective, GFP-inspired fluorescent Zn 2+ sensor is developed for two-photon microscopy and related biological application that features an 8-methoxyquinoline moiety. Excellent photophysical characteristics including a 37-fold fluorescence enhancement with excitation and emission maxima at 440 nm and 505 nm, respectively, as well as a high two-photon cross-section of 73 GM at 880 nm are reported. Based on the experimental data, the relationship between the structure and properties was elucidated and explained backed up by DFT calculations, particularly the observed PeT phenomenon for the turn-on process. Biological validation and detailed experimental and theoretical characterization of the free and the zinc-bound compounds are presented.
Keyphrases
- living cells
- single molecule
- photodynamic therapy
- label free
- fluorescent probe
- density functional theory
- heavy metals
- computed tomography
- electronic health record
- molecular dynamics
- molecular dynamics simulations
- light emitting
- monte carlo
- high resolution
- big data
- high throughput
- machine learning
- energy transfer
- loop mediated isothermal amplification
- real time pcr
- pet imaging
- oxide nanoparticles