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Genome assembly and annotation of the European earwig Forficula auricularia (subspecies B).

Upendra Raj BhattaraiMandira KatuwalRobert PoulinNeil J GemmellEddy J Dowle
Published in: G3 (Bethesda, Md.) (2022)
The European earwig Forficula auricularia is an important model for studies of maternal care, sexual selection, sociality, and host-parasite interactions. However, detailed genetic investigations of this species are hindered by a lack of genomic resources. Here, we present a high-quality hybrid genome assembly for Forficula auricularia using Nanopore long-reads and 10× linked-reads. The final assembly is 1.06 Gb in length with 31.03% GC content. It consists of 919 scaffolds with an N50 of 12.55 Mb. Half of the genome is present in only 20 scaffolds. Benchmarking Universal Single-Copy Orthologs scores are ∼90% from 3 sets of single-copy orthologs (eukaryotic, insect, and arthropod). The total repeat elements in the genome are 64.62%. The MAKER2 pipeline annotated 12,876 protein-coding genes and 21,031 mRNAs. Phylogenetic analysis revealed the assembled genome as that of species B, one of the 2 known genetic subspecies of Forficula auricularia. The genome assembly, annotation, and associated resources will be of high value to a large and diverse group of researchers working on dermapterans.
Keyphrases
  • genome wide
  • healthcare
  • copy number
  • mental health
  • mass spectrometry
  • pregnant women
  • gene expression
  • single cell
  • body mass index
  • chronic pain
  • high resolution
  • plasmodium falciparum
  • tandem mass spectrometry