Logic-Gated Proximity Aptasensing for Cell-Surface Real-Time Monitoring of Apoptosis.
Jiao ZhengQiwei WangLin ShiPai PengLili ShiTao LiPublished in: Angewandte Chemie (International ed. in English) (2021)
In nature, intact apoptotic cells release ATP as a signaling molecule to trigger prompt phagocytic clearance, even at the earliest stage of apoptosis. Inspired by this, here we introduce a straightforward strategy for real-time monitoring ATP exocytosis and drug-stimulated apoptosis in the cancer cell surroundings. Triplex-boosted G-quadruplexes (tb-G4s) responding to cell environmental factors (H+ and K+ ) are engineered to construct a DNA logic-gated nanoplatform for proximity ATP aptasensing on the cell surface. It enables the real-time monitoring of cell apoptosis by capturing released endogenous ATP during chemotherapy drug stimulation, providing a sensitive approach for dynamically evaluating drug-induced apoptosis and therapeutic efficacy.
Keyphrases
- induced apoptosis
- endoplasmic reticulum stress
- cell surface
- oxidative stress
- cell cycle arrest
- cell death
- signaling pathway
- pi k akt
- adverse drug
- cell proliferation
- mycobacterium tuberculosis
- photodynamic therapy
- emergency department
- single cell
- squamous cell carcinoma
- drug induced
- locally advanced
- radiation therapy