Lycopene Scavenges Cellular ROS, Modulates Autophagy and Improves Survival through 7SK snRNA Interaction in Smooth Muscle Cells.
Ayed A ShatiRefaat A EidMohamed Samir Ahmed ZakiYoussef A AlqahtaniSaleh M Al-QahtaniHarish C ChandramoorthyPublished in: Cells (2022)
The chance of survival rate and autophagy of smooth muscle cells under calcium stress were drastically improved with a prolonged inclusion of Lycopene in the media. The results showed an improved viability from 41% to 69% and a reduction in overall autophagic bodies from 7% to 3%, which was well in agreement with the LC3II and III mRNA levels. However, the proliferation was slow compared to the controls. The fall in the major inflammatory marker TNF-α and improved antioxidant enzyme GPx were regarded as significant restoration markers of cell survival. The reactive oxygen species (ROS) were reduced from 8 fold to 3 fold post addition of lycopene for 24 h. Further, the docking studies revealed binding of lycopene molecules with 7SK snRNA at 7.6 kcal/mol docking energy with 300 ns stability under physiological conditions. Together, these results suggest that Lycopene administration during ischemic heart disease might improve the functions of the smooth muscle cells and 7SK snRNA might be involved in the binding of lycopene and its antioxidant protective effects.
Keyphrases
- cell death
- reactive oxygen species
- oxidative stress
- signaling pathway
- molecular dynamics
- dna damage
- molecular dynamics simulations
- endoplasmic reticulum stress
- rheumatoid arthritis
- binding protein
- anti inflammatory
- protein protein
- zika virus
- small molecule
- free survival
- simultaneous determination
- dengue virus
- single cell
- transcription factor
- liquid chromatography