Non-Productive Infection of Glial Cells with SARS-CoV-2 in Hamster Organotypic Cerebellar Slice Cultures.
Lise LamoureuxBabu SajeshJessy A SlotaSarah J MedinaMatthew MayorKathy L FrostBryce WarnerKathy ManguiatHeidi WoodDarwyn KobasaStephanie A BoothPublished in: Viruses (2022)
The numerous neurological syndromes associated with COVID-19 implicate an effect of viral pathogenesis on neuronal function, yet reports of direct SARS-CoV-2 infection in the brain are conflicting. We used a well-established organotypic brain slice culture to determine the permissivity of hamster brain tissues to SARS-CoV-2 infection. We found levels of live virus waned after inoculation and observed no evidence of cell-to-cell spread, indicating that SARS-CoV-2 infection was non-productive. Nonetheless, we identified a small number of infected cells with glial phenotypes; however, no evidence of viral infection or replication was observed in neurons. Our data corroborate several clinical studies that have assessed patients with COVID-19 and their association with neurological involvement.
Keyphrases
- sars cov
- respiratory syndrome coronavirus
- cerebral ischemia
- induced apoptosis
- white matter
- cell cycle arrest
- resting state
- coronavirus disease
- single cell
- cell therapy
- neuropathic pain
- spinal cord
- endoplasmic reticulum stress
- magnetic resonance
- cell death
- machine learning
- emergency department
- stem cells
- computed tomography
- multiple sclerosis
- big data
- artificial intelligence
- cell proliferation
- pi k akt
- drug induced
- deep learning