Detection of mecA- and mecC-Positive Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates by the New Xpert MRSA Gen 3 PCR Assay.
Karsten BeckerOlivier DenisSandrine RoisinAlexander MellmannEvgeny A IdelevichDennis KnaackSarah van AlenAndré KriegeskorteRobin KöckFrieder SchaumburgGeorg PetersBritta BallhausenPublished in: Journal of clinical microbiology (2015)
An advanced methicillin-resistant Staphylococcus aureus (MRSA) detection PCR approach targeting SCCmec-orfX along with mecA and mecC was evaluated for S. aureus and coagulase-negative staphylococci. The possession of mecA and/or mecC was correctly confirmed in all cases. All methicillin-susceptible S. aureus strains (n = 98; including staphylococcal cassette chromosome mec element [SCCmec] remnants) and 98.1% of the MRSA strains (n = 160, including 10 mecC-positive MRSA) were accurately categorized.