Rapid method for simultaneous determination of ascorbic acid and zinc in effervescent tablets by capillary zone electrophoresis with contactless conductivity detection.
Michelle Miranda A C RibeiroAliceana A PradoAlex Domingues BatistaRodrigo Alejandro Abarza MunozEduardo Mathias RichterPublished in: Journal of separation science (2019)
Ascorbic acid and zinc are essential nutrients that play important roles in nutrition, immune support, and maintenance of health. For this reason, both compounds are widely used as ingredients in dietary supplements. We report, for the first time, an analytical method for fast simultaneous determination of ascorbic acid and zinc. A single analysis run is possible every 80 s (45 injections/h). The method is based on capillary electrophoresis with capacitively coupled contactless conductivity detection using a fused silica capillary with 50 cm length (effective length of 10 cm). The separation was achieved by using a background electrolyte composed by 30 mmol/L of 2-(morpholin-4-yl)ethane-1-sulfonic acid and 30 mmol/L of histidine, pH 6.1. The detection limits were 10 and 20 μmol/L and recovery values for spiked samples were 101 and 100% for zinc and ascorbic acid, respectively. The results obtained with the developed procedure were compared to those obtained by titration (ascorbic acid) and flame atomic absorption spectroscopy (zinc), and no statistically significant differences were observed (95% confidence level).
Keyphrases
- simultaneous determination
- capillary electrophoresis
- liquid chromatography
- mass spectrometry
- liquid chromatography tandem mass spectrometry
- loop mediated isothermal amplification
- oxide nanoparticles
- tandem mass spectrometry
- high performance liquid chromatography
- ultra high performance liquid chromatography
- solid phase extraction
- high resolution mass spectrometry
- public health
- label free
- healthcare
- high resolution
- physical activity
- mental health
- heavy metals
- ionic liquid
- climate change
- sensitive detection
- social media
- solid state