Real-time quantitative PCR assay development and application for assessment of agricultural surface water and various fecal matter for prevalence of Aliarcobacter faecis and Aliarcobacter lanthieri.
Mary G MiltenburgMichel CloutierEmilia CraiovanDavid R LapenGraham WilkesEdward ToppIzhar U H KhanPublished in: BMC microbiology (2020)
The results indicate that the occurrence of these species in agricultural surface water is potentially due to fecal contamination of water from livestock, human, or wildlife as both species were detected in fecal samples. The new real-time qPCR assays can facilitate rapid and accurate detection in < 3 h to quantify total numbers of A. faecis and A. lanthieri cells present in various complex environmental samples.
Keyphrases
- risk assessment
- human health
- heavy metals
- loop mediated isothermal amplification
- induced apoptosis
- climate change
- high throughput
- endothelial cells
- high resolution
- real time pcr
- cell cycle arrest
- risk factors
- health risk
- genetic diversity
- cell death
- oxidative stress
- endoplasmic reticulum stress
- single cell
- sensitive detection
- quantum dots