Fast Protein Modification in the Nanomolar Concentration Range Using an Oxalyl Amide as Latent Thioester.
Benoît SnellaBenjamin GrainJérôme VicogneFrédéric CapetBirgit WiltschiOleg MelnykVangelis AgouridasPublished in: Angewandte Chemie (International ed. in English) (2022)
We show that latent oxalyl thioester surrogates are a powerful means to modify peptides and proteins in highly dilute conditions in purified aqueous media or in mixtures as complex as cell lysates. Designed to be shelf-stable reagents, they can be activated on demand to enable ligation reactions with peptide concentrations as low as a few hundred nM at rates approaching 30 M -1 s -1 .