Cu single-atom catalyst-based flexible hydrogen peroxide electrochemical sensor with oxygen resistance for monitoring ROS bursts.
Meihong PengJing JiangShutong ChenKai LiYu-Qing LinPublished in: The Analyst (2023)
The study of cellular responses linked to oxidative stress mechanisms is crucial in comprehending diverse physiological and pathological life processes, including mitochondrial dysfunction. Nonetheless, despite the interference of O 2 , the monitoring of ROS released from cells poses a challenging task. In this study, carbon-based copper single-atom catalysts (Cu SACs) were synthesized that exhibits excellent electrocatalytic performance for H 2 O 2 reduction with an initial potential at 0.23 V and effectively avoids interference from O 2 . Based on this catalyst, a flexible and stretchable oxygen-tolerant sensor was constructed and applied to monitor the calcium ion-induced ROS burst in human umbilical vein endothelial cells (HUVECs) in a simulated physiological condition. This study effectively eradicates interference that may arise from the reduction of O 2 and presents a dependable platform for real-time in situ monitoring of physiologically active molecules by utilizing H 2 O 2 detection.
Keyphrases
- hydrogen peroxide
- oxidative stress
- dna damage
- endothelial cells
- cell death
- induced apoptosis
- highly efficient
- gold nanoparticles
- reactive oxygen species
- high throughput
- molecular dynamics
- cell proliferation
- room temperature
- mass spectrometry
- high frequency
- endoplasmic reticulum stress
- cell cycle arrest
- human health
- liquid chromatography
- simultaneous determination
- heat shock