Evaluation and statistical optimization of a method for methylated cell-free fetal DNA extraction from maternal plasma.

Mostafa AkbariqomiReza HeidariSoraya Saleh GargariMir Davood OmraniGarshasb RigiNafiseh Sadat SanikhaniHamid KooshkiFatemeh MahmoudianMohammad Ali MazlomiGholamreza Tavoosidana

Published in: Journal of assisted reproduction and genetics (2019)

Our results indicate that the THPG method is more efficient than the other tested methods for extraction of low copy number methylated cffDNA from a small volume of maternal plasma.

Keyphrases

cell free
copy number
mitochondrial dna
circulating tumor
birth weight
genome wide
pregnancy outcomes
dna methylation
pregnant women
gestational age
single molecule
body mass index
circulating tumor cells