Exhaled breath condensate in acute pulmonary embolism; a porcine study of effect of condensing temperature and feasibility of protein analysis by mass spectrometry.
Inger Lise GadeJacob Gammelgaard SchultzLasse Jørgensen CehofskiBenedict KjaergaardMarianne Tang SeverinsenBodil Steen RasmussenHenrik VorumBent HonoréSøren Risom KristensenPublished in: Journal of breath research (2020)
The EBC from pigs with acute intermediate-high-risk PE contained sufficient amounts of protein for analysis by nLC-MS/MS. The proteins were from relevant cellular compartments, indicating that EBC is a possible source for biomarkers for acute PE.
Keyphrases
- pulmonary embolism
- liver failure
- respiratory failure
- mass spectrometry
- ms ms
- aortic dissection
- drug induced
- inferior vena cava
- hepatitis b virus
- liquid chromatography
- high resolution
- protein protein
- intensive care unit
- amino acid
- binding protein
- liquid chromatography tandem mass spectrometry
- high resolution mass spectrometry
- tandem mass spectrometry
- ultra high performance liquid chromatography