Exploring Subcellular Cerebellar Fractions with the Electron Microscope.

Published in: Cerebellum (London, England) (2022)

Differential ultracentrifugation and subcellular fractionation historically helped to study the components of the cell, to discover new cellular organelles, and to decipher their morphological and molecular properties. In neuroscience, the technique has yielded important results on neuron biochemistry and the mechanisms of synaptic transmission. This Cerebellar Classic is devoted to the pioneering work of Manuel del Cerro, Ray S. Snider, and Mary Lou Oster-Granite, who isolated purified fractions after successive centrifugations of the rat cerebellum from birth to adulthood and studied them under the electron microscope.

Keyphrases

single cell
oxidative stress
cell therapy
depressive symptoms
single molecule
early life
electron transfer
solid state