Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II.
Raymond G SierraCornelius GatiHartawan LaksmonoE Han DaoSheraz GulFranklin FullerJan KernRuchira ChatterjeeMohamed IbrahimAaron S BrewsterIris D YoungTara Michels-ClarkAndrew AquilaMengning LiangMark S HunterJason E KoglinSébastien BoutetElia A JuncoBrandon HayesMichael J BoganChristina Y HamptonElisabetta V PuglisiNicholas K SauterClaudiu A StanAthina ZouniJunko YanoVittal K YachandraS Michael SoltisJoseph D PuglisiHasan DeMirciPublished in: Nature methods (2015)
We describe a concentric-flow electrokinetic injector for efficiently delivering microcrystals for serial femtosecond X-ray crystallography analysis that enables studies of challenging biological systems in their unadulterated mother liquor. We used the injector to analyze microcrystals of Geobacillus stearothermophilus thermolysin (2.2-Å structure), Thermosynechococcus elongatus photosystem II (<3-Å diffraction) and Thermus thermophilus small ribosomal subunit bound to the antibiotic paromomycin at ambient temperature (3.4-Å structure).