Login / Signup

Next-Generation Sequencing (NGS) in COVID-19: A Tool for SARS-CoV-2 Diagnosis, Monitoring New Strains and Phylodynamic Modeling in Molecular Epidemiology.

Goldin JohnNikhil Shri SahajpalAshis K MondalSudha AnanthColin WilliamsAlka ChaubeyAmyn M RojianiRavindra Kolhe
Published in: Current issues in molecular biology (2021)
This review discusses the current testing methodologies for COVID-19 diagnosis and explores next-generation sequencing (NGS) technology for the detection of SARS-CoV-2 and monitoring phylogenetic evolution in the current COVID-19 pandemic. The review addresses the development, fundamentals, assay quality control and bioinformatics processing of the NGS data. This article provides a comprehensive review of the obstacles and opportunities facing the application of NGS technologies for the diagnosis, surveillance, and study of SARS-CoV-2 and other infectious diseases. Further, we have contemplated the opportunities and challenges inherent in the adoption of NGS technology as a diagnostic test with real-world examples of its utility in the fight against COVID-19.
Keyphrases
  • sars cov
  • respiratory syndrome coronavirus
  • quality control
  • infectious diseases
  • coronavirus disease
  • electronic health record
  • copy number
  • public health
  • big data
  • deep learning
  • single cell
  • real time pcr