Photolithographic Fabrication of Semi 3-D Microstructures Composed of Flexible Hydrogel Sheet for in Vivo -like Cell Culture System.
Atsushi TsuyukuboKimio SumaruToshiyuki KanamoriPublished in: ACS applied bio materials (2019)
Insufficient reliability of current drug screening by cell-based assay has been one of the factors in the poor success rate in drug development. To improve the situation, we proposed a cell culture system using semi 3-D hydrogel microstructures as cell culture scaffolds. Because of its flexibility and permeability, the microstructure was expected to enhance the physiological function of cells. We developed a simple method of fabricating the unique hydrogel microstructures composed of hydroxypropyl cellulose through photolithography. Functionalization with poly(styrene- co -maleic anhydride) gained their cell adhesion, and it was demonstrated that several kinds of cells were incubated on the cell culture scaffolds.
Keyphrases
- tissue engineering
- induced apoptosis
- drug delivery
- cell adhesion
- cell cycle arrest
- hyaluronic acid
- endoplasmic reticulum stress
- wound healing
- high throughput
- signaling pathway
- emergency department
- oxidative stress
- white matter
- ionic liquid
- multiple sclerosis
- cell death
- cell proliferation
- mesenchymal stem cells
- bone marrow
- drug induced
- adverse drug