Bio-metal-organic frameworks for molecular recognition and sorbent extraction of hydrophilic vitamins followed by their determination using HPLC-UV.
Héctor Martínez Pérez-CejuelaMarta MonJesus Ferrando SoriaEmilio PardoDonatella ArmentanoErnesto Francisco Simó-AlfonsoJosé Manuel Herrero-MartinezPublished in: Mikrochimica acta (2020)
A bio-metal-organic framework (bio-MOF) derived from the amino acid L-serine has been prepared in bulk form and evaluated as sorbent for the molecular recognition and extraction of B-vitamins. The functional pores of bio-MOF exhibit high amounts of hydroxyl groups jointly directing other supramolecular host-guest interactions thus providing the recognition of B-vitamins in fruit juices and energy drinks. Single-crystal X-ray diffraction studies reveal the specific B-vitamin binding sites and the existence of multiple hydrogen bonds between these target molecules and the framework. It offered unique snapshots to accomplish an efficient capture of these solutes in complex aqueous matrices. Four B-vitamins (thiamin, nicotinic acid, nicotinamide, and pyridoxine) were investigated. They were eluted from the sorbent with phosphate buffer at pH 7 and analyzed by HPLC with UV detection. The sorbent was compared with commercial C18 cartridges. Following the procedure, acceptable reproducibility (RSD values < 14%) was achieved, and the detection limits were in the range 0.4 to 1.4 ng mL-1. The method was applied to the analysis of energy drink and juice samples and the recoveries were between 75 and 123% in spiked beverage samples. Graphical abstractA bio-MOF as SPE sorbent was prepared and applied to the extraction of B-vitamins in fruit juices and energy drinks.
Keyphrases
- metal organic framework
- solid phase extraction
- ms ms
- high performance liquid chromatography
- molecularly imprinted
- simultaneous determination
- liquid chromatography
- tandem mass spectrometry
- amino acid
- mass spectrometry
- high resolution
- real time pcr
- computed tomography
- gene expression
- gas chromatography
- magnetic resonance
- label free
- dna methylation
- case control
- energy transfer
- high speed