Benzovindiflupyr has gained increasing attention as a new chiral succinate dehydrogenase inhibitor fungicide; however, its determination, bioactivity, and mechanism at the enantiomeric level are very limited. In the present study, optical rotation determination and X-ray single-crystal diffraction results identified that the absolute configurations were (+)-(1 R ,4 S )-benzovindiflupyr and (-)-(1 S ,4 R )-benzovindiflupyr. A quantitative determination method for enantiomers was established using high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) for pesticide detection. The stereoselective bioactivity assay indicated that (-)-(1 S ,4 R )-benzovindiflupyr exhibited greater potency than (+)-(1 R ,4 S )-benzovindiflupyr against seven phytopathogenic fungi. Molecular docking analysis showed that (-)-(1 S ,4 R )-benzovindiflupyr possessed a stronger binding affinity to succinate dehydrogenase than (+)-(1 R ,4 S )-benzovindiflupyr. The binding modes between enantiomers and the mutant with H272(B) predicted that the phytopathogenic fungi with H272(B) of succinate dehydrogenase mutation would not be resistant to benzovindiflupyr enantiomers. This study provides a basis for residue evaluation, risk assessment, and the safe application of benzovindiflupyr at the enantiomer level.
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