A monomeric StayGold fluorescent protein.

Esther Ivorra-Molla Dipayan Akhuli Martin B L McAndrew William T Scott Lokesh Kumar Saravanan Palani Masanori Mishima Allister Crow Mohan K Balasubramanian

Published in: Nature biotechnology (2023)

StayGold is an exceptionally bright and stable fluorescent protein that is highly resistant to photobleaching. Despite favorable fluorescence properties, use of StayGold as a fluorescent tag is limited because it forms a natural dimer. Here we report the 1.6 Å structure of StayGold and generate a derivative, mStayGold, that retains the brightness and photostability of the original protein while being fully monomeric.

Keyphrases

quantum dots
living cells
protein protein
amino acid
binding protein
single molecule
small molecule