Sperm chromatin structure assay (SCSA ® ) and flow cytometry-assisted TUNEL assay provide a concordant assessment of sperm DNA fragmentation as a function of age in a large cohort of approximately 10,000 patients.
Paria BehdarvandianAli Nasr-EsfahaniMarziyeh TavalaeeKosar PashaeiNushin NaderiZahra DarmishonnejadJorge HallakRobert J AitkenParviz GharagozlooJoël R DrevetMohammad Hossein Nasr-EsfahaniPublished in: Basic and clinical andrology (2023)
In the large cohort analyzed, SDF is an age-dependent parameter, increasing linearly with aging. The SCSA® assessment of SDF and the flow cytometry-assisted TUNEL assessment are well correlated, although TUNEL is less sensitive than SCSA®. This difference in sensitivity should be taken into account in the final assessment of the true level of fragmentation of the sperm nucleus of a given sample. The classical sperm parameters (motility, morphology, sperm count) do not change dramatically with age, making them inadequate to assess the fertility potential of an individual.
Keyphrases
- flow cytometry
- end stage renal disease
- gene expression
- newly diagnosed
- ejection fraction
- dna damage
- chronic kidney disease
- peritoneal dialysis
- transcription factor
- oxidative stress
- risk assessment
- single molecule
- prognostic factors
- dna methylation
- cystic fibrosis
- pseudomonas aeruginosa
- patient reported outcomes
- young adults
- childhood cancer