In silico analysis revealing CsrA roles in motility-sessility switching and tuning VBNC cells in Vibrio parahaemolyticus.
Dan WangSteve H FlintDragana GagicJon S PalmerGraham C FletcherStephen L W OnPublished in: Biofouling (2021)
The formation of biofilms is a survival strategy employed by bacteria to help protect them from changing or unfavourable environments. In this research, 319 genes which govern biofilm formation in V. parahaemolyticus, as reported in 1,625 publications, were analysed using protein-protein-interaction (PPI) network analysis. CsrA was identified as a motility-sessility switch and biofilm formation regulator. Through robust rank aggregation (RRA) analysis of GSE65340, the generation of viable but non-culturable (VBNC) cells that may enhance cell tolerance to stress, was found to be associated with the TCA cycle and carbon metabolism biological pathways. The finding that CsrA is likely to play a role in the development of VBNC cells improves understanding of the molecular mechanisms of VBNC formation in V. parahaemolyticus and contributes to on-going efforts to reduce the hazard posed by this foodborne pathogen.
Keyphrases
- biofilm formation
- candida albicans
- pseudomonas aeruginosa
- staphylococcus aureus
- induced apoptosis
- escherichia coli
- protein protein
- cell cycle arrest
- network analysis
- small molecule
- cystic fibrosis
- genome wide
- pi k akt
- bone marrow
- quality improvement
- transcription factor
- gene expression
- cell therapy
- molecular docking
- cell proliferation