A new anti-vitiligo unsaturated fatty glycoside from Euphorbia humifusa Willd.

A new unsaturated fatty glycoside Humionoactosides A, together with four known compounds were isolated from the whole plant of Euphorbia humifusa Willd. Their structures were identified by MS/MS, NMR and ECD spectroscopic analyses, and comparison with the literature data. All compounds were evaluated for the effect on melanogenesis and tyrosinase activity use B16 cells in vitro , and the results showed that compound 1 could significantly improve the formation of melanin (138.7%, 50  μ M) and tyrosinase activity (137.2%, 50  μ M) compared with the positive control 8-MOP (125.2%, 138.9%, 50  μ M) in a dose-dependent manner. This is the first time that those compounds reported from Euphorbia humifusa Willd. with potential anti-vitiligo activity.