Phytochemical Profiling and Antibacterial Activity of Methanol Leaf Extract of Skimmia anquetilia .

Skimmia anquetilia is a plant species native to the Western Himalaya region that has tremendous potential for phytochemical activities. This study aimed to identify bioactive compounds and assess the antibacterial activity of S. anquetilia . To determine the major bioactive chemicals in the methanol leaf extract of S. anquetilia , we used a gas chromatography-mass spectrometer (GC-MS). The presence of 35 distinct phytoconstituents was discovered using GC-MS, which could contribute to the therapeutic capabilities of this plant species. The most predominant compound was 2R-acetoxymethyl-1,3,3-trimethyl-4t-(3-methyl-2-buten-1-yl)-1t-cyclohexanol (23.9%). Further, the leaf extract was evaluated for antibacterial activity against Pseudomonas aeruginosa , Escherichia coli , Klebsiella pneumoniae , Salmonella typhi , and Staphylococcus aureus . The extract showed the highest zone of inhibition against E. coli (19 mm) followed by P. aeruginosa (18 mm) and K. Pneumoniae (17 mm) at 160 mg mL -1 . The minimum inhibitory concentration (MIC) of methanol extract against the strain of P. aeruginosa (2 mg mL -1 ) demonstrated significant antibacterial activity. The findings of the present study highlight the potential of S. anquetilia for the development of herbal medicines for the treatment of various pathogenic infections.