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Structure of the SARS-Unique Domain C From the Bat Coronavirus HKU4.

Andrew J StaupIvon U De SilvaJustin T CattXuan TanRobert G HammondMargaret A Johnson
Published in: Natural product communications (2019)
Coronaviruses (CoVs) that cause infections such as severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome phylogenetically originate from bat CoVs. The coronaviral nonstructural protein 3 (nsp3) has been implicated in viral replication, polyprotein cleavage, and host immune interference. We report the structure of the C domain from the SARS-Unique Domain of bat CoV HKU4. The protein has a frataxin fold, consisting of 5 antiparallel β strands packed against 2 α helices. Bioinformatics analyses and nuclear magnetic resonance experiments were conducted to investigate the function of HKU4 C. The results showed that HKU4 C engages in protein-protein interactions with the nearby M domain of nsp3. The HKU4 C residues involved in protein-protein interactions are conserved in group 2c CoVs, indicating a conserved function.
Keyphrases
  • protein protein
  • sars cov
  • magnetic resonance
  • small molecule
  • transcription factor
  • case report
  • magnetic resonance imaging
  • amino acid
  • computed tomography
  • dna binding
  • binding protein
  • respiratory tract