DRUG RESISTANCE PATTERN AND MOLECULAR CHARACTERIZATION OF MYCOBACTERIUM TUBERCULOSIS STRAINS IN PUNJAB, PAKISTAN.
Hasnain JavedHafiza Jawairia JashmiZarfishan TahirNazia JamilPublished in: The Southeast Asian journal of tropical medicine and public health (2018)
Tuberculosis (TB) is a cause of death from a single infectious agent Mycobacterium
tuberculosis (MTB), leading to approximately 2.5 million deaths annually
worldwide. Information regarding prevalence and pattern of drug resistance
among TB patients in Pakistan remains inadequate due to the country’s limited
resources. This study compared conventional diagnostic techniques with a PCRbased
assay targeting IS6110 sequence. In addition, MTB drug resistant profiles
against four first-line drugs (ethambutol, isoniazid, rifampin, and streptomycin)
from new and retreatment cases of TB. From 101 sputum samples microscopic
examination of Ziehl-Neelsen-stained smears and culturing on Lowenstein Jensen
medium resulted in 96% and 100% positives, compared to 98% by PCR. Prevalence
of MDR-MTB was 41.5% and 58.5% among new (n = 51) and retreatment
(n = 50) cases, but 10% of the former group were sensitive to all four first-line
anti-TB drugs. Thus, MDR-MTB is highly prevalent among TB patients in Punjab
Province, Pakistan (where the study was conducted) and, although PCR amplification
of MTB-specific IS6110 sequence was rapid, it lacked the sensitivity of
the culture assay.
Keyphrases
- mycobacterium tuberculosis
- pulmonary tuberculosis
- drug resistant
- end stage renal disease
- multidrug resistant
- ejection fraction
- newly diagnosed
- chronic kidney disease
- peritoneal dialysis
- escherichia coli
- cystic fibrosis
- pseudomonas aeruginosa
- patient reported outcomes
- single cell
- drug delivery
- health information
- patient reported
- cancer therapy
- hiv aids