Evaluation of a pan-Leishmania SL RNA qPCR assay for parasite detection in laboratory-reared and field-collected sand flies and reservoir hosts.

Myrthe PareynRik HendrickxNigatu GirmaSarah HendrickxLieselotte Van BockstalNatalie Van HoutteSimon ShibruLouis MaesHerwig LeirsGuy Caljon

Published in: Parasites & vectors (2020)

This study shows that a crude extraction method in combination with the SL RNA qPCR assay is suitable for the detection and quantification of Leishmania in sand flies. The assay is inexpensive, sensitive and pan-Leishmania specific, and accordingly an excellent assay for high-throughput screening in entomological research.

Keyphrases

high throughput
drinking water
loop mediated isothermal amplification
drosophila melanogaster
sensitive detection
nucleic acid
plasmodium falciparum