Evaluation of the MDR Direct Flow Chip Kit for the Detection of Multiple Antimicrobial Resistance Determinants.
Ángel Rodríguez-VillodresAntonio Galiana-CabreraIgnacio Torres FinkRosario Duran JiménezJosé Miguel CisnerosJosé Antonio LepePublished in: Microbial drug resistance (Larchmont, N.Y.) (2023)
The objective of this study was to evaluate the accuracy of the MDR Direct Flow Chip Kit for the detection of antimicrobial resistance (AMR) determinants from bacterial colonies. Ninety-two clinical isolates with known AMR determinants genotypically characterized were used. The MDR Direct Flow Chip Kit is a microarray-based assay that included 55 AMR determinants for beta-lactams (23), quinolones (13), aminoglycosides (5), macrolides (5), sulfonamides (3), colistin (2), vancomycin (2), chloramphenicol (1), and linezolid (1). The MDR Direct Flow Chip Kit correctly detects 52 of 53 AMR determinants tested. The cfr gene (linezolid resistance) was not detected. The global sensibility, specificity, positive predictive value, and the negative predictive value calculated were 98%, 100%, 100%, and 97%. The Cohen's Kappa coefficient calculated was 0.97 [95% Confidence Interval (0.90-1.03)]. In conclusion, the MDR Direct Flow Chip is an accurate assay for the detection of multiple AMR determinants in one simple reaction.
Keyphrases
- antimicrobial resistance
- high throughput
- multidrug resistant
- circulating tumor cells
- drug resistant
- loop mediated isothermal amplification
- methicillin resistant staphylococcus aureus
- escherichia coli
- acinetobacter baumannii
- gram negative
- label free
- magnetic resonance
- klebsiella pneumoniae
- real time pcr
- transcription factor
- immune response
- cystic fibrosis
- genome wide identification