The cleavage of sialic acids by neuraminidase (NA) facilitates the spread of influenza A virus (IV) descendants. Understanding the enzymatic activity of NA aids research into the transmission of IVs. An effective method for purifying NA was developed using p -aminophenyloxamic acid-modified functionalized hydroxylated magnetic particles (AAMPs), and from 0.299 to 0.401 mg of NA from eight IV strains was isolated by 1 mg AAMP. A combination of lectin microarrays and MALDI-TOF/TOF-MS was employed to investigate the N -glycans of isolated NAs. We found that more than 20 N -glycans were identified, and 16 glycan peaks were identical in the strains derived from chicken embryo cultivation. Multi-antennae, bisected, or core-fucosylated N -glycans are common in all the NAs. The terminal residues of N -glycans are predominantly composed of galactose and N -acetylglucosamine residues. Meanwhile, sialic acid residue was uncommon in these N -glycans. Further computational docking analysis predicted the interaction mechanism between NA and p -aminophenyloxamic acid.