Anti-Aging Effect and Mechanism of Proanthocyanidins Extracted from Sea buckthorn on Hydrogen Peroxide-Induced Aging Human Skin Fibroblasts.
Xinying LiuYi XingMichael YuenTina YuenHywel YuenQiang PengPublished in: Antioxidants (Basel, Switzerland) (2022)
Oxidative stress is the leading cause of skin aging damage. Excessive accumulation of reactive oxygen species (ROS) in cells induced by hydrogen peroxide (H 2 O 2 ) triggers a decrease in collagen synthesis and an increase in collagen degradation, which are biomarkers of skin aging. We evaluated the potential protective mechanism of Sea buckthorn proanthocyanidins (SBP) against the oxidative stress-induced skin aging process from multiple aspects. We treated human skin fibroblasts (HSFs) with 300 µmoL/L of H 2 O 2 for 24 h, followed by 25, 50, and 100 µg/mL of SBP for 24 h. The results showed that SBP could enhance the activities of superoxide dismutase (SOD) and glutathione (GSH), effectively remove excess ROS, and significantly improve the changes in cell morphology and viability caused by excessive ROS in skin cells. In addition, SBP could promote the synthesis of Col I in aging HSFs through the TGF-β1/Smads pathway and inhibit the degradation of Col I by regulating the MMPs/TIMPs system, thereby maintaining the stability of the ECM structure to achieve anti-aging purposes. Finally, we studied the migration ability of SBP, and the results showed that 100 µg/mL of SBP was most conducive to the cell migration of senescent cells, laying a foundation for follow-up animal experiments. These results will increase the application value of SBP in the cosmetic and antioxidative functional food industries.
Keyphrases
- hydrogen peroxide
- induced apoptosis
- reactive oxygen species
- oxidative stress
- cell cycle arrest
- wound healing
- cell death
- dna damage
- nitric oxide
- soft tissue
- cell migration
- endoplasmic reticulum stress
- signaling pathway
- diabetic rats
- extracellular matrix
- single cell
- stem cells
- risk assessment
- physical activity
- bone marrow
- weight gain
- high glucose
- human health