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Detection of cofilin mRNA by hybridization-sensitive double-stranded fluorescent probes.

Ha Jung LeeGui Han GoJong Jin RoByeang Hyean Kim
Published in: RSC advances (2018)
We have developed hybridization-sensitive fluorescent oligonucleotide probes that, in the presence of quencher strands, undergo efficient fluorescence quenching through the formation of partial DNA/DNA duplexes. In the presence of target RNA, rapid displacement of the quencher strands results in highly enhanced fluorescence.
Keyphrases
  • single molecule
  • living cells
  • nucleic acid
  • loop mediated isothermal amplification
  • label free
  • quantum dots
  • binding protein
  • energy transfer
  • circulating tumor
  • sensitive detection
  • cell free
  • photodynamic therapy