Detection of cofilin mRNA by hybridization-sensitive double-stranded fluorescent probes.
Ha Jung LeeGui Han GoJong Jin RoByeang Hyean KimPublished in: RSC advances (2018)
We have developed hybridization-sensitive fluorescent oligonucleotide probes that, in the presence of quencher strands, undergo efficient fluorescence quenching through the formation of partial DNA/DNA duplexes. In the presence of target RNA, rapid displacement of the quencher strands results in highly enhanced fluorescence.