ᶫ-Leucine Loading and Release in MIL-100 Nanoparticles.
Ivan E GorbanMikhail A SoldatovVera V ButovaPavel V MedvedevOlga A BurachevskayaAnna BelanovaPeter ZolotukhinAlexander V SoldatovPublished in: International journal of molecular sciences (2020)
Synthesis of the MIL-100 metal-organic framework particles was carried out by hydrothermal (HT) and microwave (MW)-assisted methods. Transmission electron microscopy showed formation of microparticles in the course of hydrothermal synthesis and nanoparticles for microwave-assisted synthesis. Powder X-ray diffraction confirmed formation of larger crystallites for hydrothermal synthesis. Particle aggregation in aqueous solution was observed by dynamic light scattering. However, the stability of both samples could be improved in acetic acid solution. Nitrogen sorption isotherms showed high porosity of the particles. ᶫ-leucine molecule was used as a model molecule for loading in the porous micro- and nanoparticles. Loading was estimated by FTIR spectroscopy and thermogravimetric analysis. UV-VIS spectroscopy quantified ᶫ-leucine release from the particles in aqueous solution. Cytotoxicity studies using the HeLa cell model showed that the original particles were somewhat toxic, but ᶫ-leucine loading ameliorated the toxic effects, likely due to signaling properties of the amino acid.
Keyphrases
- aqueous solution
- metal organic framework
- electron microscopy
- sewage sludge
- high resolution
- amino acid
- anaerobic digestion
- stem cells
- single cell
- mass spectrometry
- municipal solid waste
- magnetic resonance
- cell therapy
- solid phase extraction
- walled carbon nanotubes
- computed tomography
- mesenchymal stem cells
- risk assessment
- highly efficient
- cell death
- heavy metals
- data analysis
- radiofrequency ablation
- contrast enhanced
- pi k akt