Accelerated single cell seeding in relapsed multiple myeloma.
Heather J LandauVenkata YellapantulaBenjamin T DiamondEven Holth RustadKylee H MaclachlanGunes GundemJuan Medina-MartinezJuan Arango OssaMax Fine LevineYangyu ZhouRajya KappagantulaPriscilla BaezMarc A AttiyehAlvin Makohon-MooreLance ZhangEileen M BoyleTimothy Cody AshbyPatrick BlaneyMinal PatelYanming ZhangAhmet DoganDavid J ChungSergio GiraltOscar B LahoudJonathan U PeledMichael ScordoGunjan ShahHani HassounNeha S KordeAlexander M LesokhinSydney LuSham MailankodyUrvi A ShahEric SmithMalin L HultcrantzGary A UlanerFrits van RheeGareth J MorganCarl Ola LandgrenElli PapaemmanuilChristine A Iacobuzio-DonahueFrancesco MauraPublished in: Nature communications (2020)
Multiple myeloma (MM) progression is characterized by the seeding of cancer cells in different anatomic sites. To characterize this evolutionary process, we interrogated, by whole genome sequencing, 25 samples collected at autopsy from 4 patients with relapsed MM and an additional set of 125 whole exomes collected from 51 patients. Mutational signatures analysis showed how cytotoxic agents introduce hundreds of unique mutations in each surviving cancer cell, detectable by bulk sequencing only in cases of clonal expansion of a single cancer cell bearing the mutational signature. Thus, a unique, single-cell genomic barcode can link chemotherapy exposure to a discrete time window in a patient's life. We leveraged this concept to show that MM systemic seeding is accelerated at relapse and appears to be driven by the survival and subsequent expansion of a single myeloma cell following treatment with high-dose melphalan therapy and autologous stem cell transplant.
Keyphrases
- multiple myeloma
- single cell
- high dose
- rna seq
- stem cells
- high throughput
- cell therapy
- end stage renal disease
- newly diagnosed
- genome wide
- acute lymphoblastic leukemia
- low dose
- ejection fraction
- chronic kidney disease
- acute myeloid leukemia
- stem cell transplantation
- diffuse large b cell lymphoma
- case report
- prognostic factors
- dna methylation
- patient reported outcomes
- squamous cell carcinoma
- locally advanced
- copy number
- bone marrow
- gene expression
- combination therapy