Endoplasmic Reticulum-Targeted Ratiometric N-Heterocyclic Carbene Borane Probe for Two-Photon Microscopic Imaging of Hypochlorous Acid.
Yen Leng PakSang Jun ParkGyeongok SongYubin YimHyuk KangHwan Myung KimJean BouffardJuyoung YoonPublished in: Analytical chemistry (2018)
The naphthoimidazolium borane 4 is shown to be a selective probe for HOCl over other reactive oxygen species. Unlike other boronate-reactive oxygen species (ROS) fluorogenic probes that are oxidized by HOCl through a nucleophilic borono-Dakin oxidation mechanism, probe 4 is distinguished by its electrophilic oxidation mechanism involving B-H bond cleavage. Two-photon microscopy experiments in living cells and tissues with the probe 4 demonstrate the monitoring of endogenous HOCl generation and changes in HOCl concentrations generated in the endoplasmic reticulum during oxidative stress situations.
Keyphrases
- living cells
- endoplasmic reticulum
- reactive oxygen species
- fluorescent probe
- single molecule
- oxidative stress
- high resolution
- hydrogen peroxide
- dna damage
- cell death
- gene expression
- cancer therapy
- quantum dots
- dna binding
- transcription factor
- mass spectrometry
- ischemia reperfusion injury
- electron transfer
- label free
- endoplasmic reticulum stress