Detailed Characterization of Antipathogenic Properties of Human Milk N -Glycome, against Staphylococcus aureus , Indicating Its Targeting on Cell Surface Proteins.

Human milk N -glycome was previously identified to have strong antipathogenic activities. This study is aimed to characterize the detailed antibacterial properties and the potential function mechanism of human milk N -glycome against Staphylococcus aureus . A serials of traditional antibacterial assays showed that human milk N -glycome possessed both bacteriostatic and bactericidal activities, which was further confirmed by the cell structure disruption including the change of transmembrane potential and leakage of intracellular contents. The results of the bacterial surface zeta potential and hydrophobicity, bacterial binding assay, gel shift assay, and fluorescence spectra and the different synergistic effects of human milk N -glycome combined with different antibiotics indicated that the bacterial surface proteins could be the targets of human milk N -glycome. Moreover, human milk N -glycome also showed antibiofilm activity. In conclusion, human milk N -glycome exhibited good potential for acting as an antibacterial substance against S. aureus and the antibacterial mechanism was a cell surface targeting action.