Functional Bioinorganic Hybrids from Enzymes and Luminescent Silicon-Based Nanoparticles.
Christopher Jay T RobidilloMuhammad Amirul IslamMaryam AghajamaliAngelique FaramusRegina SinelnikovXiyu ZhangJan H van EschJonathan G-C VeinotPublished in: Langmuir : the ACS journal of surfaces and colloids (2018)
This study reports the preparation of functional bioinorganic hybrid materials exhibiting catalytic activity and photoluminescent properties arising from the combination of enzymes and freestanding silicon-based nanoparticles. The hybrid materials reported herein have potential applications in biological sensing/imaging and theranostics, as they combine long-lived silicon-based nanoparticle photoluminescence with substrate-specific enzymatic activity. Thermal hydrosilylation of undecenoic acid and alkene-terminated poly(ethylene oxide) with hydride-terminated silicon nanocrystals afforded nanoparticles functionalized with a mixed surface made up of carboxylic acid and poly(ethylene oxide) moieties. These silicon-based nanoparticles were subsequently conjugated with prototypical enzymes through the carbodiimide-mediated amide coupling reaction in order to form bioinorganic hybrids that display solubility and photostability in phosphate buffer, photoluminescence (λmax = 630 nm), and enzymatic activity. They were characterized using Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM), dynamic light scattering analysis (DLS), photoluminescence spectroscopy, and pertinent enzyme activity assays.
Keyphrases
- quantum dots
- high resolution
- energy transfer
- electron microscopy
- photodynamic therapy
- single molecule
- sensitive detection
- hydrogen peroxide
- light emitting
- magnetic resonance imaging
- walled carbon nanotubes
- molecularly imprinted
- emergency department
- nitric oxide
- risk assessment
- computed tomography
- fluorescence imaging
- adverse drug
- tandem mass spectrometry