Light-Emitting-Diode-Based Multispectral Photoacoustic Computed Tomography System.
Sumit AgrawalChristopher FaddenAjay DangiXinyi YangHussain AlbahraniNeilesh FringsSara Heidari ZadiSri-Rajasekhar KothapalliPublished in: Sensors (Basel, Switzerland) (2019)
Photoacoustic computed tomography (PACT) has been widely explored for non-ionizing functional and molecular imaging of humans and small animals. In order for light to penetrate deep inside tissue, a bulky and high-cost tunable laser is typically used. Light-emitting diodes (LEDs) have recently emerged as cost-effective and portable alternative illumination sources for photoacoustic imaging. In this study, we have developed a portable, low-cost, five-dimensional (x, y, z, t, λ ) PACT system using multi-wavelength LED excitation to enable similar functional and molecular imaging capabilities as standard tunable lasers. Four LED arrays and a linear ultrasound transducer detector array are housed in a hollow cylindrical geometry that rotates 360 degrees to allow multiple projections through the subject of interest placed inside the cylinder. The structural, functional, and molecular imaging capabilities of the LED-PACT system are validated using various tissue-mimicking phantom studies. The axial, lateral, and elevational resolutions of the system at 2.3 cm depth are estimated as 0.12 mm, 0.3 mm, and 2.1 mm, respectively. Spectrally unmixed photoacoustic contrasts from tubes filled with oxy- and deoxy-hemoglobin, indocyanine green, methylene blue, and melanin molecules demonstrate the multispectral molecular imaging capabilities of the system. Human-finger-mimicking phantoms made of a bone and blood tubes show structural and functional oxygen saturation imaging capabilities. Together, these results demonstrate the potential of the proposed LED-based, low-cost, portable PACT system for pre-clinical and clinical applications.
Keyphrases
- low cost
- light emitting
- fluorescence imaging
- computed tomography
- high resolution
- photodynamic therapy
- magnetic resonance imaging
- positron emission tomography
- image quality
- endothelial cells
- low dose
- dual energy
- high throughput
- bone mineral density
- magnetic resonance
- optical coherence tomography
- postmenopausal women
- single cell
- climate change
- molecularly imprinted
- metal organic framework
- radiation therapy
- pet ct
- human health
- case control
- liquid chromatography