Optimising nucleic acid recovery from rapid antigen tests for whole genome sequencing of respiratory viruses.
G Butel-SimoesE SteinigI SavicM ZhanduisenovG PapadakisT TranJ MoselenL CalyD A WilliamsonC K LimPublished in: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology (2024)
Under simulated conditions, RAT-WGS demonstrated that (i) viral genomes were generally stable at 4°C at 72 h and 1 week, (ii) RNALater has a more significant preservation of nucleic acids compared to DNA/RNA Shield and (iii) genome recovery can be achieved using a sequencing depth of 500,000 reads per sample in RNALater, across all respiratory viruses and conditions.