Living Artificial Skin: Photosensitizer and Cell Sandwiched Bacterial Cellulose for Chronic Wound Healing.
Xingang LiuMeng WangLei CaoJiahao ZhuangDandan WangMin WuJie LiuPublished in: Advanced materials (Deerfield Beach, Fla.) (2024)
Chronic wounds pose a significant global public health challenge due to their suboptimal treatment efficacy caused by bacterial infections and microcirculatory disturbances. Inspired by the biofunctionality of natural skin, an artificial skin (HV@BC@TBG) is bioengineered with bacterial cellulose (BC) sandwiched between photosensitizers (PS) and functionalized living cells. Glucose-modified PS (TBG) and vascular endothelial growth factor (VEGF)-functionalized living cells (HV) are successively modified on each side of BC through biological metabolism and bio-orthogonal reaction. As the outermost layer, the TBG layer can generate reactive oxygen species (ROS) upon light illumination to efficiently combat bacterial infections. The HV layer is the inner layer near the diabetic wound, which servs as a living factory to continuously secrete VEGF to accelerate wound repair by promoting fibroblast proliferation and angiogenesis. The sandwiched structural artificial skin HV@BC@TBG is nontoxic, biocompatible, and demonstrated its ability to significantly accelerate the healing process of infected diabetic wounds, rendering it a promising next-generation medical therapy for chronic wound management.
Keyphrases
- wound healing
- living cells
- vascular endothelial growth factor
- fluorescent probe
- reactive oxygen species
- public health
- endothelial cells
- photodynamic therapy
- single molecule
- ionic liquid
- healthcare
- quantum dots
- type diabetes
- single cell
- dna damage
- cell death
- cell therapy
- stem cells
- signaling pathway
- oxidative stress
- blood pressure
- high resolution
- insulin resistance
- drug release
- combination therapy
- liquid chromatography