Therapeutic effects of apocynin on ovarian ischemia-reperfusion induced lung injury.
Ayhan TanyeliDerya Guzel ErdoganSelim ÇomaklıElif PolatMustafa Can GulerErsen EraslanSongul DoganayPublished in: Biotechnic & histochemistry : official publication of the Biological Stain Commission (2022)
Ovarian ischemia-reperfusion (I-R) injury may damage remote organs, including the lungs. We investigated whether apocynin, a NADPH oxidase inhibitor, might protect against ovarian I-R induced apoptosis in the lungs of rats. Bilateral ovarian I-R was induced for 3 h, then apocynin was applied at two concentrations. Lung tissue was evaluated using spectrophotometric and immunohistochemical methods. We found that I-R increased total oxidant status (TOS), oxidative stress index (OSI) and myeloperoxidase (MPO) levels, and immunostaining of nuclear factor kappa-B (NF-κB), light chain 3B (LC3B), interleukin 1-beta (IL-1β), caspase-3 and tumor necrosis factor-alpha (TNF-α), but decreased superoxide dismutase (SOD) values. Apocynin application to I-R injured rats enhanced recovery of lung tissue oxidants and improved both histology and frequency of apoptosis.
Keyphrases
- oxidative stress
- induced apoptosis
- nuclear factor
- diabetic rats
- endoplasmic reticulum stress
- toll like receptor
- signaling pathway
- high glucose
- rheumatoid arthritis
- ischemia reperfusion injury
- dna damage
- simultaneous determination
- cell death
- mass spectrometry
- inflammatory response
- hydrogen peroxide
- endothelial cells
- cell proliferation
- immune response
- pi k akt