High-performance liquid chromatography and Enzyme-Linked Immunosorbent Assay techniques for detection and quantification of aflatoxin B1 in feed samples: a comparative study.
Achenef Melaku BeyeneXiangwei DuDwayne E SchrunkSteve EnsleyWilson K RumbeihaPublished in: BMC research notes (2019)
The two tests showed very strong linearity with correlation coefficient value of > 0.99 using standard solutions. The mean recovery rate was 92.42% (with relative standard deviation (RSD) of 5.97) and 75.64% (RSD = 34.88) for HPLC and ELISA, respectively. There was no statistically significant difference in recovery rate between the two methods. There was a positive correlation (r = 0.84) between them which indicated that the two techniques can be used to detect and quantify aflatoxin B1 in feed samples. However, there were variations among replicates for the ELISA method, which shows that this method is more applicable for screening purposes.
Keyphrases
- high performance liquid chromatography
- simultaneous determination
- tandem mass spectrometry
- solid phase extraction
- mass spectrometry
- ms ms
- liquid chromatography
- monoclonal antibody
- high throughput
- magnetic resonance imaging
- loop mediated isothermal amplification
- magnetic resonance
- quantum dots
- single cell
- contrast enhanced