Online HPLC-ESI-HRMS Method for the Analysis and Comparison of Different Dissolved Organic Matter Samples.
Claudia PatriarcaJonas BergquistPer J R SjöbergLars TranvikJeffrey A HawkesPublished in: Environmental science & technology (2018)
Natural dissolved organic matter (DOM) is an ultracomplex mixture that is essential to global carbon cycling but is poorly understood because of its complexity. The most powerful tool for the DOM characterization is high-resolution mass spectrometry (HRMS) generally combined to direct infusion (DI) as sample introduction. Liquid chromatography (LC) represents a compelling alternative to DI; however, state-of-the-art techniques involve only offline LC-HRMS approaches, which have important logistical drawbacks that make DOM analysis more challenging. This study introduces a new method based on online coupling of liquid chromatography to high resolution mass spectrometry, able to overcome the disadvantages of usual approaches. It is characterized by high reproducibility (% Bray-Curtis dissimilarity among replicates ≈ 2.5%), and it reduces transient complexity and contaminant interferences, thus increasing the signal-to-noise ratio (S/N), leading to the identification of an overall larger number of formulas in the mixture. Moreover, the application of an in silico fractionation prior to the statistical analysis allows an easy, flexible, fast, and detailed comparison of DOM samples from a variety of sources with a single chromatographic run.
Keyphrases
- high resolution mass spectrometry
- liquid chromatography
- simultaneous determination
- mass spectrometry
- tandem mass spectrometry
- ultra high performance liquid chromatography
- gas chromatography
- solid phase extraction
- high performance liquid chromatography
- ms ms
- organic matter
- social media
- health information
- biofilm formation
- drinking water
- air pollution
- cerebral ischemia
- brain injury
- subarachnoid hemorrhage
- molecular docking
- low dose
- high resolution
- ionic liquid
- staphylococcus aureus
- blood brain barrier