Optimized reduced representation bisulfite sequencing reveals tissue-specific mCHH islands in maize.

Fei-Man HsuMing-Ren YenChi-Ting WangChien-Yu LinChung-Ju Rachel Wang Pao-Yang Chen

Published in: Epigenetics & chromatin (2017)

Our ROI-directed RRBS pipeline is eminently applicable to DNA methylation profiling of large genomes. Our results provide novel insights into the tissue-specific epigenomic landscapes in maize, demonstrating that DNA methylation and siRNA and chromatin accessibility constitute a critical, interdependent component that orchestrates the transition from the vegetative to the reproductive phase.

Keyphrases

dna methylation
genome wide
gene expression
single cell
dna damage
transcription factor
copy number
cancer therapy
oxidative stress
hyaluronic acid
data analysis