Reliable Detection of Somatic Mutations for Pancreatic Cancer in Endoscopic Ultrasonography-Guided Fine Needle Aspirates with Next-Generation Sequencing: Implications from a Prospective Cohort Study.

Joseph R HabibYayun ZhuLingdi YinAmmar A JavedDing DingJonathan TeniorMichael WrightSyed Z AliRichard A BurkhartWilliam BurnsChristopher L WolfgangEunji ShinJun YuJin He

Published in: Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract (2021)

Targeted NGS can reliably detect KRAS mutations from EUS-FNA samples and exhibits high KRAS mutational concordance with primary tumor and ctDNA. This suggests targeted NGS of EUS-FNA samples may enable preoperative ctDNA prognostication using digital droplet PCR and supplement diagnoses in patients with inconclusive EUS-FNA.

Keyphrases

fine needle aspiration
ultrasound guided
circulating tumor
cancer therapy
copy number
wild type
real time pcr
magnetic resonance imaging
patients undergoing
air pollution
high throughput
single cell
cell free
loop mediated isothermal amplification
label free